제품 > 항체 > 다중클론항체(pAb)

JOSD1 Rabbit pAb (A10426)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - JOSD1 Rabbit pAb (A10426)

Western blot analysis of extracts of various cell lines, using JOSD1 antibody (A10426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameJOSD1 Rabbit pAb
Catalog No.A10426
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable thiol-dependent deubiquitinase. Predicted to be involved in protein deubiquitination. Located in membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human JOSD1 (NP_055691.1).
SequenceMSCVPWKGDKAKSESLELPQAAPPQIYHEKQRRELCALHALNNVFQDSNAFTRDTLQEIFQRLSPNTMVTPHKKSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALTNVMGFIMNLPSSLCWGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGKNCELLLVVPEEVEAHQSWRTDV
Gene ID
Swiss Prot
SynonymsdJ508I15.2
Calculated MW23kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, DU145, Mouse liver, Mouse testis, Rat thymus, Rat liver, Rat testis
Cellular locationCell membrane, Cytoplasm
    ABclonal:Western blot - JOSD1 Rabbit pAb (A10426)}

    Western blot - JOSD1 Rabbit pAb (A10426)

    Western blot analysis of extracts of various cell lines, using JOSD1 antibody (A10426) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.