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KAT9/Elp3 Rabbit mAb (A0020)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KAT9/Elp3 Rabbit mAb (A0020)

Western blot analysis of various lysates using KAT9/Elp3 Rabbit mAb (A0020) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - KAT9/Elp3 Rabbit mAb (A0020)

Immunohistochemistry analysis of paraffin-embedded rat brain using KAT9/Elp3 Rabbit mAb (A0020) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KAT9/Elp3 Rabbit mAb (A0020)

Immunohistochemistry analysis of paraffin-embedded mouse testis using KAT9/Elp3 Rabbit mAb (A0020) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameKAT9/Elp3 Rabbit mAb
Catalog No.A0020
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1806
Enables acetyltransferase activity and phosphorylase kinase regulator activity. Involved in regulation of transcription by RNA polymerase II and tRNA wobble uridine modification. Located in cytosol and nucleolus. Part of elongator holoenzyme complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-547 of human KAT9/Elp3 (Q9H9T3).
SequenceMRQKRKGDLSPAELMMLTIGDVIKQLIEAHEQGKDIDLNKVKTKTAAKYGLSAQPRLVDIIAAVPPQYRKVLMPKLKAKPIRTASGIAVVAVMCKPHRCPHISFTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRNLHDALSGHTSNNIYEAVKYSERSLTKCIGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAVCESFHLAKDSGFKVVAHMMPDLPNVGLERDIEQFTEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYKSYSPSDLVELVARILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELALARMKDLGIQCRDVRTREVGIQEIHHKVRPYQVELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPTKFQHQGFGMLLMEEAERIAREEHGSGKIAVISGVGTRNYYRKIGYRLQGPYMVKMLK
Gene ID
Swiss Prot
SynonymsKAT9; KAT9/Elp3
Calculated MW62kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7, K-562, SH-SY5Y, Mouse testis, Mouse brain, Mouse kidney, Rat lung, Rat testis, Rat brain
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - KAT9/Elp3 Rabbit mAb (A0020)}

Western blot - KAT9/Elp3 Rabbit mAb (A0020)

Western blot analysis of various lysates using KAT9/Elp3 Rabbit mAb (A0020) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - KAT9/Elp3 Rabbit mAb (A0020)}

Immunohistochemistry - KAT9/Elp3 Rabbit mAb (A0020)

Immunohistochemistry analysis of paraffin-embedded rat brain using KAT9/Elp3 Rabbit mAb (A0020) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - KAT9/Elp3 Rabbit mAb (A0020)}

Immunohistochemistry - KAT9/Elp3 Rabbit mAb (A0020)

Immunohistochemistry analysis of paraffin-embedded mouse testis using KAT9/Elp3 Rabbit mAb (A0020) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)