제품 > 항체 > 다중클론항체(pAb)

KCNA1 Rabbit pAb (A16906)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KCNA1 Rabbit pAb (A16906)

Western blot analysis of various lysates, using KCNA1 Rabbit pAb (A16906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - KCNA1 Rabbit pAb (A16906)

Western blot analysis of lysates from HeLa cells, using KCNA1 Rabbit pAb (A16906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - KCNA1 Rabbit pAb (A16906)

Immunofluorescence analysis of paraffin-embedded mouse brain using KCNA1 Rabbit pAb (A16906) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKCNA1 Rabbit pAb
Catalog No.A16906
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a voltage-gated delayed potassium channel that is phylogenetically related to the Drosophila Shaker channel. The encoded protein has six putative transmembrane segments (S1-S6), and the loop between S5 and S6 forms the pore and contains the conserved selectivity filter motif (GYGD). The functional channel is a homotetramer. The N-terminus of the channel is associated with beta subunits that can modify the inactivation properties of the channel as well as affect expression levels. The C-terminus of the channel is complexed to a PDZ domain protein that is responsible for channel targeting. Mutations in this gene have been associated with myokymia with periodic ataxia (AEMK).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 426-495 of human KCNA1 (NP_000208.2).
SequenceQLLHVSSPNLASDSDLSRRSSSTMSKSEYMEIEEDMNNSIAHYRQVNIRTANCTTANQNCVNKSKLLTDV
Gene ID
Swiss Prot
SynonymsEA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1; KCNA1
Calculated MW56kDa
Observed MW60kDa/75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Mouse brain, Mouse spinal cord, Rat brain
Cellular locationCell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Multi-pass membrane protein, Perikaryon, axon, dendrite, presynaptic cell membrane, synapse.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - KCNA1 Rabbit pAb (A16906)}

    Western blot - KCNA1 Rabbit pAb (A16906)

    Western blot analysis of various lysates, using KCNA1 Rabbit pAb (A16906) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - KCNA1 Rabbit pAb (A16906)}

    Western blot - KCNA1 Rabbit pAb (A16906)

    Western blot analysis of lysates from HeLa cells, using KCNA1 Rabbit pAb (A16906) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - KCNA1 Rabbit pAb (A16906)}

    Immunofluorescence - KCNA1 Rabbit pAb (A16906)

    Immunofluorescence analysis of paraffin-embedded mouse brain using KCNA1 Rabbit pAb (A16906) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)