제품 > 항체 > 다중클론항체(pAb)

KCNAB1 Rabbit pAb (A15334)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KCNAB1 Rabbit pAb (A15334)

Western blot analysis of various lysates using KCNAB1 Rabbit pAb (A15334) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameKCNAB1 Rabbit pAb
Catalog No.A15334
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member includes distinct isoforms which are encoded by alternatively spliced transcript variants of this gene. Some of these isoforms are beta subunits, which form heteromultimeric complexes with alpha subunits and modulate the activity of the pore-forming alpha subunits.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 282-401 of human KCNAB1 (NP_751891.1).
SequenceCGIISGKYGNGVPESSRASLKCYQWLKERIVSEEGRKQQNKLKDLSPIAERLGCTLPQLAVAWCLRNEGVSSVLLGSSTPEQLIENLGAIQVLPKMTSHVVNEIDNILRNKPYSKKDYRS
Gene ID
Swiss Prot
SynonymshKvb3; AKR6A3; KCNA1B; Kvb1.3; hKvBeta3; KV-BETA-1; KCNAB1
Calculated MW47kDa
Observed MW45-50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-251MG, 293T, Mouse brain, Mouse kidney, Mouse heart, Rat brain
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein
    ABclonal:Western blot - KCNAB1 Rabbit pAb (A15334)}

    Western blot - KCNAB1 Rabbit pAb (A15334)

    Western blot analysis of various lysates using KCNAB1 Rabbit pAb (A15334) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)