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KCNAB2 Rabbit pAb (A16224)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - KCNAB2 Rabbit pAb (A16224)

Western blot analysis of various lysates using KCNAB2 Rabbit pAb (A16224) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - KCNAB2 Rabbit pAb (A16224)

Immunofluorescence analysis of L929 cells using KCNAB2 Rabbit pAb (A16224) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKCNAB2 Rabbit pAb
Catalog No.A16224
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member is one of the beta subunits, which are auxiliary proteins associating with functional Kv-alpha subunits. This member alters functional properties of the KCNA4 gene product. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-40 of human KCNAB2 (NP_003627.1).
SequenceMYPESTTGSPARLSLRQTGSPGMIYSTRYGSPKRQLQFYR
Gene ID
Swiss Prot
SynonymsAKR6A5; KCNA2B; HKvbeta2; KV-BETA-2; HKvbeta2.1; HKvbeta2.2; KCNAB2
Calculated MW41kDa
Observed MW45kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse eye, Mouse lung, Rat lung
Cellular locationcytoskeleton, cytosol, pinceau fiber, plasma membrane, synapse

Documents

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    ABclonal:Western blot - KCNAB2 Rabbit pAb (A16224)}

    Western blot - KCNAB2 Rabbit pAb (A16224)

    Western blot analysis of various lysates using KCNAB2 Rabbit pAb (A16224) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - KCNAB2 Rabbit pAb (A16224)}

    Immunofluorescence - KCNAB2 Rabbit pAb (A16224)

    Immunofluorescence analysis of L929 cells using KCNAB2 Rabbit pAb (A16224) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)