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KCNH1 Rabbit pAb (A6636)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KCNH1 Rabbit pAb (A6636)

Western blot analysis of extracts of various cell lines, using KCNH1 antibody (A6636) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameKCNH1 Rabbit pAb
Catalog No.A6636
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 813-962 of human KCNH1 (NP_002229.1).
SequenceFKDACGKSEDWNKVSKAESMETLPERTKASGEATLKKTDSCDSGITKSDLRLDNVGEARSPQDRSPILAEVKHSFYPIPEQTLQATVLEVRHELKEDIKALNAKMTNIEKQLSEILRILTSRRSSQSPQELFEISRPQSPESERDIFGAS
Gene ID
Swiss Prot
SynonymsEAG; EAG1; ZLS1; hEAG; TMBTS; h-eag; hEAG1; Kv10.1
Calculated MW111kDa
Observed MW111kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRaji, MCF7, HeLa
Cellular locationCell membrane, Early endosome membrane, Multi-pass membrane protein, Nucleus inner membrane

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    ABclonal:Western blot - KCNH1 Rabbit pAb (A6636)}

    Western blot - KCNH1 Rabbit pAb (A6636)

    Western blot analysis of extracts of various cell lines, using KCNH1 antibody (A6636) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)