제품 > 항체 > 다중클론항체(pAb)

KCNJ15 Rabbit pAb (A15282)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KCNJ15 Rabbit pAb (A15282)

Western blot analysis of various lysates using KCNJ15 Rabbit pAb (A15282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)

Immunohistochemistry analysis of paraffin-embedded rat heart using KCNJ15 Rabbit pAb (A15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using KCNJ15 Rabbit pAb (A15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using KCNJ15 Rabbit pAb (A15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameKCNJ15 Rabbit pAb
Catalog No.A15282
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Eight transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 221-375 of human KCNJ15 (NP_002234.2).
SequenceVTKEGERILLNQATVKFHVDSSSESPFLILPMTFYHVLDETSPLRDLTPQNLKEKEFELVVLLNATVESTSAVCQSRTSYIPEEIYWGFEFVPVVSLSKNGKYVADFSQFEQIRKSPDCTFYCADSEKQQLEEKYRQEDQRERELRTLLLQQSNV
Gene ID
Swiss Prot
SynonymsIRKK; KIR1.3; KIR4.2; KCNJ15
Calculated MW43kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse liver, Mouse kidney, Rat liver
Cellular locationMembrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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    ABclonal:Western blot - KCNJ15 Rabbit pAb (A15282)}

    Western blot - KCNJ15 Rabbit pAb (A15282)

    Western blot analysis of various lysates using KCNJ15 Rabbit pAb (A15282) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)}

    Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)

    Immunohistochemistry analysis of paraffin-embedded rat heart using KCNJ15 Rabbit pAb (A15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)}

    Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)

    Immunohistochemistry analysis of paraffin-embedded human breast cancer using KCNJ15 Rabbit pAb (A15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)}

    Immunohistochemistry - KCNJ15 Rabbit pAb (A15282)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney using KCNJ15 Rabbit pAb (A15282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)