제품 > 항체 > 다중클론항체(pAb)

KCNS2 Rabbit pAb (A15684)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KCNS2 Rabbit pAb (A15684)

Western blot analysis of lysates from rat brain, using KCNS2 Rabbit pAb (A15684) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - KCNS2 Rabbit pAb (A15684)

Immunofluorescence analysis of paraffin-embedded rat eye using KCNS2 Rabbit pAb (A15684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KCNS2 Rabbit pAb (A15684)

Immunofluorescence analysis of paraffin-embedded mouse eye using KCNS2 Rabbit pAb (A15684) at dilution of 1:100 (20x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KCNS2 Rabbit pAb (A15684)

Immunofluorescence analysis of paraffin-embedded mouse eye using KCNS2 Rabbit pAb (A15684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKCNS2 Rabbit pAb
Catalog No.A15684
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable voltage-gated potassium channel activity. Predicted to be involved in potassium ion transmembrane transport and regulation of delayed rectifier potassium channel activity. Predicted to be located in perinuclear region of cytoplasm and plasma membrane. Predicted to be part of voltage-gated potassium channel complex. Predicted to be integral component of membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human KCNS2 (NP_065748.1).
SequenceMTGQSLWDVSEANVEDGEIRINVGGFKRRLRSHTLLRFPETRLGRLLLCHSREAILELCDDYDDVQREFYFDRNPELFPYVLHFYHTGKLHVMAELCVFSFSQEIEYWGINEFFIDSCCSYSYHGRKVEPEQEKWDEQSDQESTTSSFDEILAFYNDASKFDGQPLGNFRRQLWLALDNPGYSVLSR
Gene ID
Swiss Prot
SynonymsKV9.2; KCNS2
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationCell membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - KCNS2 Rabbit pAb (A15684)}

    Western blot - KCNS2 Rabbit pAb (A15684)

    Western blot analysis of lysates from rat brain, using KCNS2 Rabbit pAb (A15684) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - KCNS2 Rabbit pAb (A15684)}

    Immunofluorescence - KCNS2 Rabbit pAb (A15684)

    Immunofluorescence analysis of paraffin-embedded rat eye using KCNS2 Rabbit pAb (A15684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - KCNS2 Rabbit pAb (A15684)}

    Immunofluorescence - KCNS2 Rabbit pAb (A15684)

    Immunofluorescence analysis of paraffin-embedded mouse eye using KCNS2 Rabbit pAb (A15684) at dilution of 1:100 (20x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - KCNS2 Rabbit pAb (A15684)}

    Immunofluorescence - KCNS2 Rabbit pAb (A15684)

    Immunofluorescence analysis of paraffin-embedded mouse eye using KCNS2 Rabbit pAb (A15684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)