제품 > 항체 > 다중클론항체(pAb)

KCNU1 Rabbit pAb (A14967)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - KCNU1 Rabbit pAb (A14967)

Western blot analysis of lysates from rat testis, using KCNU1 Rabbit pAb (A14967) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameKCNU1 Rabbit pAb
Catalog No.A14967
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the potassium channel family of proteins. The encoded voltage-gated ion channel allows the outward flow of potassium ions during plasma membrane hyperpolarization in sperm. Opening of this channel may be regulated by calcium ion levels. Homozygous knockout mice that lack the related mouse gene exhibit male sterility. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 920-1149 of human KCNU1 (NP_001027006.2).
SequenceLELLQMLVTGGVSSQLEQHLDKDKVYGVADSCTSLLSGRNRCKLGLLSLHETILSDVNPRNTFGQLFCGSLDLFGILCVGLYRIIDEEELNPENKRFVITRPANEFKLLPSDLVFCAIPFSTACYKRNEEFSLQKSYEIVNKASQTTETHSDTNCPPTIDSVTETLYSPVYSYQPRTNSLSFPKQIAWNQSRTNSIISSQIPLGDNAKENERKTSDEVYDEDPFAYSEPL
Gene ID
Swiss Prot
SynonymsKCa5; Slo3; KCNMC1; KCa5.1; Kcnma3; SPGF79; KCNU1
Calculated MW130kDa
Observed MW130kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat testis
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

IHC(Sus scrofa)

    ABclonal:Western blot - KCNU1 Rabbit pAb (A14967)}

    Western blot - KCNU1 Rabbit pAb (A14967)

    Western blot analysis of lysates from rat testis, using KCNU1 Rabbit pAb (A14967) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A14967? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)