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[KD Validated] GPLD1 Rabbit pAb (A21781)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KD Validated] GPLD1 Rabbit pAb (A21781)

Western blot analysis of lysates from Daudi cells, using [KD Validated] GPLD1 Rabbit pAb (A21781) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KD Validated] GPLD1 Rabbit pAb (A21781)

Western blot analysis of lysates from wild type(WT) and GPLD1 knockdown (KD) 293T cells, using [KD Validated] GPLD1 Rabbit pAb (A21781) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KD Validated] GPLD1 Rabbit pAb
Catalog No.A21781
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).
SequenceCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSA
Gene ID
Swiss Prot
SynonymsPLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1; [KD Validated] GPLD1
Calculated MW92kDa
Observed MW92kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Daudi
Cellular locationSecreted.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] GPLD1 Rabbit pAb (A21781)}

Western blot - [KD Validated] GPLD1 Rabbit pAb (A21781)

Western blot analysis of lysates from Daudi cells, using [KD Validated] GPLD1 Rabbit pAb (A21781) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KD Validated] GPLD1 Rabbit pAb (A21781)}

Western blot - [KD Validated] GPLD1 Rabbit pAb (A21781)

Western blot analysis of lysates from wild type(WT) and GPLD1 knockdown (KD) 293T cells, using [KD Validated] GPLD1 Rabbit pAb (A21781) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)