Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of various lysates, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and KMT5A/SETD8 knockdown (KD) 293T cells, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Enhanced Kit (RM00021).
Exposure time: 30s.
| Product name | [KD Validated] KMT5A/SETD8 Rabbit mAb |
|---|---|
| Catalog No. | A4136 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0916 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KMT5A/SETD8 (Q9NQR1). |
|---|---|
| Sequence | MGEGGAAAALVAAAAAAAAAAAAVVAGQRRRRLGRRARCHGPGRAAGGKMSKPCAVEAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | SET8; SET07; SETD8; PR-Set7; PR/SET07; [KO/KD Validated] KMT5A/SETD8 |
| Calculated MW | 43kDa |
| Observed MW | 49kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T, HeLa, U-87MG, Mouse brain, Mouse heart |
| Cellular location | Chromosome, Nucleus |
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* For research use only. Not for therapeutic or diagnostic purposes.
![ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A4136_ARC0916_WB_01.jpg?t=1714026976 "ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)")
![ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A4136_ARC0916_KO-WB_01.jpg?t=1714026976 "ABclonal:Western blot - [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136)")
