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KMT5A/SETD8 Rabbit pAb (A5256)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KMT5A/SETD8 Rabbit pAb (A5256)

Western blot analysis of various lysates using (A5256) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - KMT5A/SETD8 Rabbit pAb (A5256)

Western blot analysis of various lysates using (A5256) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)

Immunofluorescence analysis of C6 cells using KMT5A/KMT5A/SETD8 Rabbit pAb (A5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)

Immunofluorescence analysis of NIH-3T3 cells using KMT5A/KMT5A/SETD8 Rabbit pAb (A5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)

Immunofluorescence analysis of U-2 OS cells using KMT5A/KMT5A/SETD8 Rabbit pAb (A5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for KMT5A/SETD8 Rabbit pAb(A5256)

Overview

Product nameKMT5A/SETD8 Rabbit pAb
Catalog No.A5256
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human KMT5A/KMT5A/SETD8 (Q9NQR1).
SequenceMGEGGAAAALVAAAAAAAAAAAAVVAGQRRRRLGRRARCHGPGRAAGGKMSKPCAVEAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMSPNKCSGMRFPLQEENSVTHHEVKCQGKPLAGIYRKREEKRNAGNAVRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKTPPSSCDSTNAAIAKQALKKPIKGKQAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELIESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVPHLILIASRDIAAGEELLYDYGDRSKASIEAHPWLKH
Gene ID
Swiss Prot
SynonymsSET8; SET07; SETD8; PR-Set7; PR/SET07; KMT5A/SETD8
Calculated MW43kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Jurkat, U-87MG, Mouse heart, Rat lung
Cellular locationChromosome, Nucleus.
Customer validation

(HCT116)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - KMT5A/SETD8 Rabbit pAb (A5256)}

Western blot - KMT5A/SETD8 Rabbit pAb (A5256)

Western blot analysis of various lysates using (A5256) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - KMT5A/SETD8 Rabbit pAb (A5256)}

Western blot - KMT5A/SETD8 Rabbit pAb (A5256)

Western blot analysis of various lysates using (A5256) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)}

Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)

Immunofluorescence analysis of C6 cells using KMT5A/KMT5A/SETD8 Rabbit pAb (A5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)}

Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)

Immunofluorescence analysis of NIH-3T3 cells using KMT5A/KMT5A/SETD8 Rabbit pAb (A5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)}

Immunofluorescence - KMT5A/SETD8 Rabbit pAb (A5256)

Immunofluorescence analysis of U-2 OS cells using KMT5A/KMT5A/SETD8 Rabbit pAb (A5256) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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