제품 > 항체 > 단일클론항체(mAb)

[KD Validated] LAP3 Rabbit mAb (A27188)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)

Western blot analysis of lysates from wild type (WT) and LAP3 knockdown (KD) 293T cells using [KD Validated] LAP3 Rabbit mAb (A27188) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)

Western blot analysis of lysates from Hep G2 cells using [KD Validated] LAP3 Rabbit mAb (A27188) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)

Western blot analysis of various lysates using [KD Validated] LAP3 Rabbit mAb (A27188) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] LAP3 Rabbit mAb (A27188) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] LAP3 Rabbit mAb (A27188) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] LAP3 Rabbit mAb (A27188) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name[KD Validated] LAP3 Rabbit mAb
Catalog No.A27188
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC71182
Predicted to enable peptidase activity. Predicted to be involved in proteolysis. Located in cytosol; midbody; and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-180 of human LAP3 (NP_056991.2).
SequenceRFGSRSLSTADMTKGLVLGIYSKEKEDDVPQFTSAGENFDKLLAGKLRETLNISGPPLKAGKTRTFYGLHQDFPSVVLVGLGKKAAGIDEQENWHEGKENIRAAVAAGCRQIQDLELSSVEVDPCGDAQAAAEGAVLGLYEYDDLKQKKKMAVSAKLYGS
Gene ID
Swiss Prot
SynonymsLAP; PEPS; LAPEP; HEL-S-106
Calculated MW56kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:20000
  • IHC-P 1:2000 - 1:20000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHep G2, Mouse kidney, Mouse liver, Rat liver, 293T
Cellular locationCytoplasm.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)}

Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)

Western blot analysis of lysates from wild type (WT) and LAP3 knockdown (KD) 293T cells using [KD Validated] LAP3 Rabbit mAb (A27188) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)}

Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)

Western blot analysis of lysates from Hep G2 cells using [KD Validated] LAP3 Rabbit mAb (A27188) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)}

Western blot - [KD Validated] LAP3 Rabbit mAb (A27188)

Western blot analysis of various lysates using [KD Validated] LAP3 Rabbit mAb (A27188) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)}

Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] LAP3 Rabbit mAb (A27188) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)}

Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] LAP3 Rabbit mAb (A27188) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)}

Immunohistochemistry - [KD Validated] LAP3 Rabbit mAb (A27188)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] LAP3 Rabbit mAb (A27188) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)