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LAP3 Rabbit pAb (A24554)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LAP3 Rabbit pAb (A24554)

Western blot analysis of various lysates, using LAP3 Rabbit pAb (A24554) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - LAP3 Rabbit pAb (A24554)

Western blot analysis of lysates from wild type (WT) and LAP3 knockdown (KD) 293T(KD) cells, using LAP3 Rabbit pAb (A24554) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - LAP3 Rabbit pAb (A24554)

Immunofluorescence analysis of paraffin-embedded Human colon using LAP3 Rabbit pAb(A24554) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LAP3 Rabbit pAb (A24554)

Immunofluorescence analysis of paraffin-embedded Human stomach using LAP3 Rabbit pAb(A24554) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLAP3 Rabbit pAb
Catalog No.A24554
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant protein of human LAP3.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsLAP3; HEL-S-106; LAP; LAPEP; PEPS; cytosol aminopeptidase
Calculated MW52kDa/56kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, Hep G2, Mouse liver, Mouse kidney, Rat liver
Cellular locationCytoplasm

Documents

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ABclonal:Western blot - LAP3 Rabbit pAb (A24554)}

Western blot - LAP3 Rabbit pAb (A24554)

Western blot analysis of various lysates, using LAP3 Rabbit pAb (A24554) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - LAP3 Rabbit pAb (A24554)}

Western blot - LAP3 Rabbit pAb (A24554)

Western blot analysis of lysates from wild type (WT) and LAP3 knockdown (KD) 293T(KD) cells, using LAP3 Rabbit pAb (A24554) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - LAP3 Rabbit pAb (A24554)}

Immunofluorescence - LAP3 Rabbit pAb (A24554)

Immunofluorescence analysis of paraffin-embedded Human colon using LAP3 Rabbit pAb(A24554) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LAP3 Rabbit pAb (A24554)}

Immunofluorescence - LAP3 Rabbit pAb (A24554)

Immunofluorescence analysis of paraffin-embedded Human stomach using LAP3 Rabbit pAb(A24554) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)