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[KD Validated] LYPLA1 Rabbit mAb (A26848)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] LYPLA1 Rabbit mAb (A26848)

Western blot analysis of various lysates using LYPLA1 Rabbit mAb (A26848) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KD Validated] LYPLA1 Rabbit mAb (A26848)

Western blot analysis of lysates from wild type (WT) and LYPLA1 knockdown (KD) 293T cells using LYPLA1 Rabbit mAb (A26848) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] LYPLA1 Rabbit mAb (A26848)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] LYPLA1 Rabbit mAb (A26848) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name[KD Validated] LYPLA1 Rabbit mAb
Catalog No.A26848
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC71341
This gene encodes a member of the alpha/beta hydrolase superfamily. The encoded protein functions as a homodimer, exhibiting both depalmitoylating as well as lysophospholipase activity, and may be involved in Ras localization and signaling. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 4, 6, and 7.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human LYPLA1 (NP_006321.1).
SequenceMCGNNMSTPLPAIVPAARKATAAVIFLHGLGDTGHGWAEAFAGIRSSHIKYICPHAPVRPVTLNMNVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRASFPQGPIGGANRDISILQCHGDCDPLVPLMFGSLTVEKLKTLVNPANVTFKTYEGMMHSSCQQEMMDVKQFIDKLLPPID
Gene ID
Swiss Prot
SynonymsAPT1; LPL1; APT-1; LPL-I; hAPT1
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:60000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, HAP1, Mouse kidney, Mouse liver, Rat liver, Rat kidney
Cellular locationcytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, nuclear membrane, plasma membrane, Cytoplasm.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KD Validated] LYPLA1 Rabbit mAb (A26848)}

    Western blot - [KD Validated] LYPLA1 Rabbit mAb (A26848)

    Western blot analysis of various lysates using LYPLA1 Rabbit mAb (A26848) at 1:10000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - [KD Validated] LYPLA1 Rabbit mAb (A26848)}

    Western blot - [KD Validated] LYPLA1 Rabbit mAb (A26848)

    Western blot analysis of lysates from wild type (WT) and LYPLA1 knockdown (KD) 293T cells using LYPLA1 Rabbit mAb (A26848) at 1:10000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - [KD Validated] LYPLA1 Rabbit mAb (A26848)}

    Immunohistochemistry - [KD Validated] LYPLA1 Rabbit mAb (A26848)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] LYPLA1 Rabbit mAb (A26848) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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