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[KD Validated] MRPL13 Rabbit pAb (A24779)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] MRPL13 Rabbit pAb (A24779)

Western blot analysis of various lysates using [KD Validated] MRPL13 Rabbit pAb (A24779) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KD Validated] MRPL13 Rabbit pAb (A24779)

Western blot analysis of lysates from wild type (WT) and MRPL13 knockdown (KD) HeLa cells using [KD Validated] MRPL13 Rabbit pAb (A24779) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KD Validated] MRPL13 Rabbit pAb (A24779)

Immunohistochemistry analysis of MRPL13 in paraffin-embedded mouse lung tissue using [KD Validated] MRPL13 Rabbit pAb (A24779) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] MRPL13 Rabbit pAb (A24779)

Immunohistochemistry analysis of MRPL13 in paraffin-embedded human colon carcinoma tissue using [KD Validated] MRPL13 Rabbit pAb (A24779) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name[KD Validated] MRPL13 Rabbit pAb
Catalog No.A24779
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 70-178 of human MRPL13 (NP_054797.2).
SequenceNKWEQKVYSSHTGYPGGFRQVTAAQLHLRDPVAIVKLAIYGMLPKNLHRRTMMERLHLFPDEYIPEDILKNLVEELPQPRKIPKRLDEYTQEEIDAFPRLWTPPEDYRL
Gene ID
Swiss Prot
SynonymsMRPL13; L13; L13A; L13mt; RPL13; RPML13; mitochondrial ribosomal protein L13
Calculated MW20kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Mouse heart, Rat kidney, Rat heart
Cellular locationMitochondrion

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] MRPL13 Rabbit pAb (A24779)}

Western blot - [KD Validated] MRPL13 Rabbit pAb (A24779)

Western blot analysis of various lysates using [KD Validated] MRPL13 Rabbit pAb (A24779) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KD Validated] MRPL13 Rabbit pAb (A24779)}

Western blot - [KD Validated] MRPL13 Rabbit pAb (A24779)

Western blot analysis of lysates from wild type (WT) and MRPL13 knockdown (KD) HeLa cells using [KD Validated] MRPL13 Rabbit pAb (A24779) at 1:2000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - [KD Validated] MRPL13 Rabbit pAb (A24779)}

Immunohistochemistry - [KD Validated] MRPL13 Rabbit pAb (A24779)

Immunohistochemistry analysis of MRPL13 in paraffin-embedded mouse lung tissue using [KD Validated] MRPL13 Rabbit pAb (A24779) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] MRPL13 Rabbit pAb (A24779)}

Immunohistochemistry - [KD Validated] MRPL13 Rabbit pAb (A24779)

Immunohistochemistry analysis of MRPL13 in paraffin-embedded human colon carcinoma tissue using [KD Validated] MRPL13 Rabbit pAb (A24779) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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