제품 > 항체 > 다중클론항체(pAb)

[KD Validated] MRPL52 Rabbit pAb (A25498)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KD Validated] MRPL52 Rabbit pAb (A25498)

Western blot analysis of lysates from wild type (WT) and MRPL52 knockdown (KD) HeLa cells using [KD Validated] MRPL52 Rabbit pAb (A25498) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Western blot - [KD Validated] MRPL52 Rabbit pAb (A25498)

Western blot analysis of lysates from U-251 MG cells using [KD Validated] MRPL52 Rabbit pAb (A25498) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:45s.

Overview

Product name[KD Validated] MRPL52 Rabbit pAb
Catalog No.A25498
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which has no bacterial homolog. Multiple transcript variants encoding different protein isoforms were identified through sequence analysis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 24-123 of human MRPL52 (NP_848026.1).
SequenceGGQWRLQQGLAANPSGYGPLTELPDWSYADGRPAPPMKGQLRRKAERETFARRVVLLSQEMDAGLQAWQLRQQKLQEEQRKQENALKPKGASLKSPLPSQ
Gene ID
Swiss Prot
SynonymsmL52; 39S ribosomal protein L52; L52mt; MRP-L52; Mrpl52; RM52
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, U-251 MG
Cellular locationMitochondrion.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - [KD Validated] MRPL52 Rabbit pAb (A25498)}

    Western blot - [KD Validated] MRPL52 Rabbit pAb (A25498)

    Western blot analysis of lysates from wild type (WT) and MRPL52 knockdown (KD) HeLa cells using [KD Validated] MRPL52 Rabbit pAb (A25498) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Western blot - [KD Validated] MRPL52 Rabbit pAb (A25498)}

    Western blot - [KD Validated] MRPL52 Rabbit pAb (A25498)

    Western blot analysis of lysates from U-251 MG cells using [KD Validated] MRPL52 Rabbit pAb (A25498) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time:45s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)