제품 > 항체 > 단일클론항체(mAb)

[KD Validated] NF2/Merlin Rabbit mAb (A23186)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using [KD Validated] NF2/Merlin Rabbit mAb (A23186) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from wild type(WT) and NF2/Merlin knockdown (KD) HeLa cells, using [KD Validated] NF2/Merlin Rabbit mAb (A23186) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KD Validated] NF2/Merlin Rabbit mAb (A23186)

Immunohistochemistry analysis of NF2/Merlin in paraffin-embedded rat brain tissue using [KD Validated] NF2/Merlin Rabbit mAb (A23186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of NF2/Merlin in paraffin-embedded human spleen tissue using [KD Validated] NF2/Merlin Rabbit mAb (A23186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of NF2/Merlin in paraffin-embedded rat kidney tissue using [KD Validated] NF2/Merlin Rabbit mAb (A23186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of NF2/Merlin in paraffin-embedded mouse testis tissue using [KD Validated] NF2/Merlin Rabbit mAb (A23186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	[KD Validated] NF2/Merlin Rabbit mAb
Catalog No.	A23186
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59903

Background

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 347-595 of human NF2/Merlin (NP_000259.1).
Sequence	EEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL
Gene ID
Swiss Prot
Synonyms	ACN; SCH; BANF; merlin-1; in
Calculated MW	70kDa
Observed MW	70-75kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	MCF7, NIH/3T3, Mouse brain, C6, HeLa
Cellular location	cortical actin cytoskeleton, cytoplasm, cytoskeleton, cytosol, early endosome, filopodium membrane, nucleolus, nucleus, perinuclear region of cytoplasm, plasma membrane, ruffle membrane