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[KD Validated] Pin1 Rabbit mAb (A22470)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] Pin1 Rabbit mAb (A22470)

Western blot analysis of lysates from wild type(WT) and Pin1 knockdown (KD) 293T cells, using [KD Validated] Pin1 Rabbit mAb (A22470) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KD Validated] Pin1 Rabbit mAb (A22470)

Western blot analysis of various lysates, using [KD Validated] Pin1 Rabbit mAb (A22470) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name[KD Validated] Pin1 Rabbit mAb
Catalog No.A22470
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57311
Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human Pin1 (NP_006212.1).
SequenceMADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE
Gene ID
Swiss Prot
SynonymsDOD; UBL5; [KD Validated] Pin1
Calculated MW18kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, 293T, NIH/3T3, Mouse brain, PC-12, Rat brain
Cellular locationCytoplasm, Nucleus, Nucleus speckle

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] Pin1 Rabbit mAb (A22470)}

Western blot - [KD Validated] Pin1 Rabbit mAb (A22470)

Western blot analysis of lysates from wild type(WT) and Pin1 knockdown (KD) 293T cells, using [KD Validated] Pin1 Rabbit mAb (A22470) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KD Validated] Pin1 Rabbit mAb (A22470)}

Western blot - [KD Validated] Pin1 Rabbit mAb (A22470)

Western blot analysis of various lysates, using [KD Validated] Pin1 Rabbit mAb (A22470) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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