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[KD Validated] RAB10 Rabbit mAb (A22746)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] RAB10 Rabbit mAb (A22746)

Western blot analysis of various lysates, using [KD Validated] RAB10 Rabbit mAb (A22746) at 1:60000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KD Validated] RAB10 Rabbit mAb (A22746)

Western blot analysis of lysates from wild type(WT) and RAB10 knockdown (KD) U-87MG cells, using [KD Validated] RAB10 Rabbit mAb (A22746) at 1:60000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - [KD Validated] RAB10 Rabbit mAb (A22746)

Confocal imaging of NIH/3T3 cells using [KD Validated] RAB10 Rabbit mAb (A22746, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KD Validated] RAB10 Rabbit mAb
Catalog No.A22746
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55653
RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 101-200 of human RAB10 (NP_057215.3).
SequenceSKWLRNIDEHANEDVERMLLGNKCDMDDKRVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC
Gene ID
Swiss Prot
SynonymsRAB10; ras-related protein Rab-10; [KD Validated] RAB10
Calculated MW23kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:70000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, U-87MG, MCF7, C2C12, C6
Cellular locationCell projection, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lipid-anchor, Recycling endosome membrane, cilium, phagosome membrane, trans-Golgi network membrane

Documents

Certificate of Compliance

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ABclonal:Western blot - [KD Validated] RAB10 Rabbit mAb (A22746)}

Western blot - [KD Validated] RAB10 Rabbit mAb (A22746)

Western blot analysis of various lysates, using [KD Validated] RAB10 Rabbit mAb (A22746) at 1:60000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KD Validated] RAB10 Rabbit mAb (A22746)}

Western blot - [KD Validated] RAB10 Rabbit mAb (A22746)

Western blot analysis of lysates from wild type(WT) and RAB10 knockdown (KD) U-87MG cells, using [KD Validated] RAB10 Rabbit mAb (A22746) at 1:60000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - [KD Validated] RAB10 Rabbit mAb (A22746)}

Immunofluorescence - [KD Validated] RAB10 Rabbit mAb (A22746)

Confocal imaging of NIH/3T3 cells using [KD Validated] RAB10 Rabbit mAb (A22746, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)