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[KD Validated] RHOT1/MIRO1 Rabbit mAb (A22469)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using [KD Validated] RHOT1/MIRO1 Rabbit mAb (A22469) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from wild type(WT) and RHOT1/MIRO1 knockdown (KD) 293T cells, using [KD Validated] RHOT1/MIRO1 Rabbit mAb (A22469) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KD Validated] RHOT1/MIRO1 Rabbit mAb (A22469)

Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] RHOT1/MIRO1 Rabbit mAb (A22469) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	[KD Validated] RHOT1/MIRO1 Rabbit mAb
Catalog No.	A22469
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57606

Background

Predicted to enable GTP binding activity and GTPase activity. Involved in cellular homeostasis; mitochondrial outer membrane permeabilization; and mitochondrion transport along microtubule. Is integral component of mitochondrial outer membrane.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1/MIRO1 (NP_060777.3).
Sequence	QSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSS
Gene ID
Swiss Prot
Synonyms	ARHT1; MIRO1; MIRO-1; [KO/KD Validated] RHOT1/MIRO1
Calculated MW	71kDa
Observed MW	75kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	K-562, 293T, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular location	Mitochondrion outer membrane, Single-pass type IV membrane protein