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[KD Validated] SRP72 Rabbit mAb (A25418)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] SRP72 Rabbit mAb (A25418)

Western blot analysis of lysates from HeLa cells transfected with SRP72 siRNA using [KD Validated] SRP72 Rabbit mAb (A25418) at 1:3000 dilution. 
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 18 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KD Validated] SRP72 Rabbit mAb (A25418)

Western blot analysis of various lysates using [KD Validated] SRP72 Rabbit mAb (A25418) at 1:3000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Human testis tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] SRP72 Rabbit mAb (A25418)

Confocal imaging of PC-12 cells using [KD Validated] SRP72 Rabbit mAb (A25418, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KD Validated] SRP72 Rabbit mAb (A25418)

Confocal imaging of HeLa cells using [KD Validated] SRP72 Rabbit mAb (A25418, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo®488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KD Validated] SRP72 Rabbit mAb
Catalog No.A25418
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63042
This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 440-544 of human SRP72 (NP_008878.3).
SequenceQPKSPAHLSLIREAANFKLKYGRKKEAISDLQQLWKQNPKDIHTLAQLISAYSLVDPEKAKALSKHLPSSDSMSLKVDVEALENSAGATYIRKKGGKVTGDSQPK
Gene ID
Swiss Prot
SynonymsBMFF; BMFS1; HEL103; SRP72
Calculated MW75kDa
Observed MW75kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:12000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, PC-12, 293T, HeLa
Cellular locationCytoplasm, Endoplasmic reticulum.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Western blot - [KD Validated] SRP72 Rabbit mAb (A25418)

    Western blot analysis of lysates from HeLa cells transfected with SRP72 siRNA using [KD Validated] SRP72 Rabbit mAb (A25418) at 1:3000 dilution. 
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 18 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Western blot - [KD Validated] SRP72 Rabbit mAb (A25418)

    Western blot analysis of various lysates using [KD Validated] SRP72 Rabbit mAb (A25418) at 1:3000 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Human testis tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunohistochemistry - [KD Validated] SRP72 Rabbit mAb (A25418)

    Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KD Validated] SRP72 Rabbit mAb (A25418) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunofluorescence - [KD Validated] SRP72 Rabbit mAb (A25418)

    Confocal imaging of PC-12 cells using [KD Validated] SRP72 Rabbit mAb (A25418, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - [KD Validated] SRP72 Rabbit mAb (A25418)}

    Immunofluorescence - [KD Validated] SRP72 Rabbit mAb (A25418)

    Confocal imaging of HeLa cells using [KD Validated] SRP72 Rabbit mAb (A25418, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo®488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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