제품 > 항체 > 다중클론항체(pAb)

[KD Validated] TFEB Rabbit pAb (A7311)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] TFEB Rabbit pAb (A7311)

Western blot analysis of various lysates, using [KD Validated] TFEB Rabbit pAb (A7311) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) HeLa cells using [KD Validated] TFEB Rabbit pAb (A7311) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KD Validated] TFEB Rabbit pAb (A7311)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] TFEB Rabbit pAb (A7311) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KD Validated] TFEB Rabbit pAb (A7311) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] TFEB Rabbit pAb (A7311) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] TFEB Rabbit pAb (A7311)

Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] TFEB Rabbit pAb (A7311) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using [KD Validated] TFEB Rabbit pAb (A7311) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using [KD Validated] TFEB Rabbit pAb (A7311) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	[KD Validated] TFEB Rabbit pAb
Catalog No.	A7311
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including cellular response to amino acid starvation; lysosome localization; and positive regulation of autophagy. Located in cytosol; lysosomal membrane; and nucleoplasm.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 50-173 of human TFEB (NP_009093.1).
Sequence	TPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGY
Gene ID
Swiss Prot
Synonyms	TCFEB; BHLHE35; ALPHATFEB; TFEB
Calculated MW	53kDa
Observed MW	65-70kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Daudi, Rat thymus, HeLa
Cellular location	Cytoplasm, Nucleus.
Customer validation	(H460) IF(Homo sapiens, Capra hircus, Mus musculus) WB(Homo sapiens, Capra hircus, Mus musculus) IF(Homo sapiens, Mus musculus) WB(Mus musculus) IHC(Mus musculus) RT-qPCR(Mus musculus) IHC(Mus musculus) Other(Mus musculus, Macaca mulatta)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구후성유전학 & 핵 신호전달전사 인자

[KD Validated] TFEB Rabbit pAb images

Western blot - [KD Validated] TFEB Rabbit pAb (A7311)

Immunohistochemistry - [KD Validated] TFEB Rabbit pAb (A7311)

Immunofluorescence - [KD Validated] TFEB Rabbit pAb (A7311)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A7311

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Cellular Signalling

Application:

IF:

4.4

Species:

Homo sapiens

PMID:

28366813

Title:

MITA modulated autophagy flux promotes cell death in breast cancer cells

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Int J Mol Sci

Application:

IF:

5.6

Species:

Capra hircus

PMID:

34070207

Title:

Essential Role of CRIM1 on Endometrial Receptivity in Goat

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Curr Eye Res

Application:

IF:

1.7

Species:

Homo sapiens

PMID:

34872443

Title:

Downregulation of AMPK dependent FOXO3 and TFEB involves in the inhibition of autophagy in diabetic cataract

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Food & function

Application:

IF:

5.1

Species:

Capra hircus

PMID:

35640273

Title:

Baicalin alleviates endometrial inflammatory injury through regulation of tight junction proteins

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Front Pharmacol

Application:

WB, IF

IF:

5.6

Species:

Homo sapiens

PMID:

34776962

Title:

SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Homo sapiens, Mus musculus

PMID:

37441529

Title:

Oat beta-glucan reduces colitis by promoting autophagy flux in intestinal epithelial cells via EPHB6-TFEB axis - PMC

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

4.2

Species:

Homo sapiens

PMID:

37087023

Title:

Regulation of self-renewal in ovarian cancer stem cells by fructose via chaperone-mediated autophagy

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Nature nanotechnology

Application:

IF, WB

IF:

38.1

Species:

Mus musculus

PMID:

38158436

Title:

Intracerebral fate of organic and inorganic nanoparticles is dependent on microglial extracellular vesicle function

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

British journal of pharmacology

Application:

IF:

7.3

Species:

Mus musculus

PMID:

37850255

Title:

A snake cathelicidin enhances transcription factor EB-mediated autophagy and alleviates ROS-induced pyroptosis after ischaemiareperfusion injury of island skin flaps

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Aging

Application:

WB, IF

IF:

3.9

Species:

Mus musculus

PMID:

33714196

Title:

PGC-1 alleviates mitochondrial dysfunction via TFEB-mediated autophagy in cisplatin-induced acute kidney injury

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Gastroenterology

Application:

WB, IHC, IF

IF:

25.7

Species:

Mus musculus

PMID:

36436593

Title:

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Neuropathology and applied neurobiology

Application:

RT-qPCR

IF:

Species:

Mus musculus

PMID:

35881686

Title:

Genetic Inhibition of PDK1 Robustly Reduces Plaque Deposition and Ameliorates Gliosis in the 5 FAD Mouse Model of Alzheimer's Disease

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF, IHC, WB

IF:

5.7

Species:

Mus musculus

PMID:

38907713

Title:

Ginsenoside-Rh2 Promotes Functional Recovery after Spinal Cord Injury by Enhancing TFEB-Mediated Autophagy

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

The Journal of cell biology

Application:

IF:

7.4

Species:

Homo sapiens

PMID:

38913026

Title:

Excessive STAU1 condensate drives mTOR translation and autophagy dysfunction in neurodegeneration

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

Other

IF:

5.7

Species:

Mus musculus

PMID:

38869217

Title:

Hesperidin Alleviates Hepatic Injury Caused by Deoxynivalenol Exposure through Activation of mTOR and AKT/GSK3/TFEB Pathways

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

Other

IF:

5.7

Species:

Macaca mulatta

PMID:

38869217

Title:

Hesperidin Alleviates Hepatic Injury Caused by Deoxynivalenol Exposure through Activation of mTOR and AKT/GSK3/TFEB Pathways

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

Other

IF:

5.7

Species:

Mus musculus

PMID:

38869217

Title:

Hesperidin Alleviates Hepatic Injury Caused by Deoxynivalenol Exposure through Activation of mTOR and AKT/GSK3/TFEB Pathways

References for [KD Validated] TFEB Rabbit pAb (A7311)

Product:

[KD Validated] TFEB Rabbit pAb

Journal:

Autophagy

Application:

IF:

14.6

Species:

Mus musculus

PMID:

38390831

Title:

TRIM27 elicits protective immunity against tuberculosis by activating TFEB-mediated autophagy flux

Publishing research using A7311? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)

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제품 > 항체 > 다중클론항체(pAb)

[KD Validated] TFEB Rabbit pAb (A7311)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

[KD Validated] TFEB Rabbit pAb images

Submit an review for A7311

Reviews List

Product Protocols

항체 (3)

Secondary Antibodies (26)