제품 > 항체 > 단일클론항체(mAb)

[KD Validated] TRIM25 Rabbit mAb (A25846)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from wild type (WT) and TRIM25 knockdown (KD) 293T cells using [KD Validated] TRIM25 Rabbit mAb (A25846) at 1:4700 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2s.

ABclonal:Immunohistochemistry - [KD Validated] TRIM25 Rabbit mAb (A25846)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] TRIM25 Rabbit mAb (A25846) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] TRIM25 Rabbit mAb (A25846)

Confocal imaging of HeLa cells using [KD Validated] TRIM25 Rabbit mAb (A25846, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KD Validated] TRIM25 Rabbit mAb (A25846)

Immunoprecipitation of TRIM25 from 300 µg extracts of 293T cells was performed using 3 µg of [KD Validated] TRIM25 Rabbit mAb (A25846). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KD Validated] TRIM25 Rabbit mAb (A25846) at a dilution of 1:5000.

Overview

Product name	[KD Validated] TRIM25 Rabbit mAb
Catalog No.	A25846
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC67400

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 190-379 of human TRIM25 (NP_005073.2).
Sequence	SLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRP
Gene ID
Swiss Prot
Synonyms	EFP; Z147; RNF147; ZNF147
Calculated MW	71kDa
Observed MW	72kDa/

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:4000 - 1:24000 IHC-P 1:300 - 1:3000 IF/ICC 1:200 - 1:800 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T,
Cellular location	Cytoplasm.