제품 > 항체 > 단일클론항체(mAb)

[KD Validated] USF1 Rabbit mAb (A23474)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KD Validated] USF1 Rabbit mAb (A23474)

Western blot analysis of lysates from PC-12 cells, using [KD Validated] USF1 Rabbit mAb (A23474) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KD Validated] USF1 Rabbit mAb (A23474)

Western blot analysis of lysates from wild type(WT) and USF1 knockdown (KD) Hela cells, using [KD Validated] USF1 Rabbit mAb (A23474) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)

Immunohistochemistry analysis of paraffin-embedded Human spleen using USF1 Rabbit mAb (A23474) at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using USF1 Rabbit mAb (A23474) at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using USF1 Rabbit mAb (A23474) at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] USF1 Rabbit mAb (A23474)

Confocal imaging of paraffin-embedded Rat thymus using [KD Validated] USF1 Rabbit mAb (A23474, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - [KD Validated] USF1 Rabbit mAb (A23474)

Confocal imaging of paraffin-embedded Mouse brain using [KD Validated] USF1 Rabbit mAb (A23474, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.

Overview

Product name[KD Validated] USF1 Rabbit mAb
Catalog No.A23474
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58148
This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1. (NP_009053.1).
SequenceMKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
Gene ID
Swiss Prot
SynonymsUEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11; [KD Validated] USF1
Calculated MW34kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IHC-P 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHela, PC-12
Cellular locationNucleus
Customer validation

IP(homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KD Validated] USF1 Rabbit mAb (A23474)}

Western blot - [KD Validated] USF1 Rabbit mAb (A23474)

Western blot analysis of lysates from PC-12 cells, using [KD Validated] USF1 Rabbit mAb (A23474) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KD Validated] USF1 Rabbit mAb (A23474)}

Western blot - [KD Validated] USF1 Rabbit mAb (A23474)

Western blot analysis of lysates from wild type(WT) and USF1 knockdown (KD) Hela cells, using [KD Validated] USF1 Rabbit mAb (A23474) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)}

Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)

Immunohistochemistry analysis of paraffin-embedded Human spleen using USF1 Rabbit mAb (A23474) at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)}

Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using USF1 Rabbit mAb (A23474) at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)}

Immunohistochemistry - [KD Validated] USF1 Rabbit mAb (A23474)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using USF1 Rabbit mAb (A23474) at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KD Validated] USF1 Rabbit mAb (A23474)}

Immunofluorescence - [KD Validated] USF1 Rabbit mAb (A23474)

Confocal imaging of paraffin-embedded Rat thymus using [KD Validated] USF1 Rabbit mAb (A23474, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - [KD Validated] USF1 Rabbit mAb (A23474)}

Immunofluorescence - [KD Validated] USF1 Rabbit mAb (A23474)

Confocal imaging of paraffin-embedded Mouse brain using [KD Validated] USF1 Rabbit mAb (A23474, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A23474? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)