Western blot - [KD Validated] VDAC3 Rabbit mAb (A26244)
Western blot analysis of various lysates using [KD Validated] VDAC3 Rabbit mAb (A26244) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Western blot - [KD Validated] VDAC3 Rabbit mAb (A26244)
Western blot analysis of lysates from wild type (WT) and VDAC3 knockdown (KD) 293T cells using [KD Validated] VDAC3 Rabbit mAb (A26244) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Western blot - [KD Validated] VDAC3 Rabbit mAb (A26244)
Western blot analysis of lysates from Rat testis using [KD Validated] VDAC3 Rabbit mAb (A26244) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
Immunohistochemistry - [KD Validated] VDAC3 Rabbit mAb (A26244)
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] VDAC3 Rabbit mAb (A26244) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunohistochemistry - [KD Validated] VDAC3 Rabbit mAb (A26244)
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] VDAC3 Rabbit mAb (A26244) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
Immunofluorescence - [KD Validated] VDAC3 Rabbit mAb (A26244)
Confocal imaging of paraffin-embedded Mouse testis tissue using [KD Validated] VDAC3 Rabbit mAb (A26244, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Immunofluorescence - [KD Validated] VDAC3 Rabbit mAb (A26244)
Confocal imaging of paraffin-embedded Rat testis tissue using [KD Validated] VDAC3 Rabbit mAb (A26244, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Immunofluorescence - [KD Validated] VDAC3 Rabbit mAb (A26244)
Confocal imaging of 293T cells using [KD Validated] VDAC3 Rabbit mAb (A26244, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.