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VDAC3 Rabbit pAb (A10544)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - VDAC3 Rabbit pAb (A10544)

Western blot analysis of lysates from Mouse heart, using VDAC3 Rabbit pAb (A10544) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - VDAC3 Rabbit pAb (A10544)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using VDAC3 Rabbit pAb (A10544) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VDAC3 Rabbit pAb (A10544)

Immunohistochemistry analysis of paraffin-embedded mouse lung using VDAC3 Rabbit pAb (A10544) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - VDAC3 Rabbit pAb (A10544)

Immunofluorescence analysis of HeLa cells using VDAC3 Rabbit pAb (A10544) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameVDAC3 Rabbit pAb
Catalog No.A10544
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a voltage-dependent anion channel (VDAC), and belongs to the mitochondrial porin family. VDACs are small, integral membrane proteins that traverse the outer mitochondrial membrane and conduct ATP and other small metabolites. They are known to bind several kinases of intermediary metabolism, thought to be involved in translocation of adenine nucleotides, and are hypothesized to form part of the mitochondrial permeability transition pore, which results in the release of cytochrome c at the onset of apoptotic cell death. Alternatively transcript variants encoding different isoforms have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-283 of human VDAC3 (NP_005653.3).
SequenceMCNTPTYCDLGKAAKDVFNKGYGFGMVKIDLKTKSCSGVEFSTSGHAYTDTGKASGNLETKYKVCNYGLTFTQKWNTDNTLGTEISWENKLAEGLKLTLDTIFVPNTGKKSGKLKASYKRDCFSVGSNVDIDFSGPTIYGWAVLAFEGWLAGYQMSFDTAKSKLSQNNFALGYKAADFQLHTHVNDGTEFGGSIYQKVNEKIETSINLAWTAGSNNTRFGIAAKYMLDCRTSLSAKVNNASLIGLGYTQTLRPGVKLTLSALIDGKNFSAGGHKVGLGFELEA
Gene ID
Swiss Prot
SynonymsVDAC-3; HD-VDAC3; VDAC3
Calculated MW31kDa
Observed MW31kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart
Cellular locationMitochondrion outer membrane
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - VDAC3 Rabbit pAb (A10544)}

Western blot - VDAC3 Rabbit pAb (A10544)

Western blot analysis of lysates from Mouse heart, using VDAC3 Rabbit pAb (A10544) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - VDAC3 Rabbit pAb (A10544)}

Immunohistochemistry - VDAC3 Rabbit pAb (A10544)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using VDAC3 Rabbit pAb (A10544) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - VDAC3 Rabbit pAb (A10544)}

Immunohistochemistry - VDAC3 Rabbit pAb (A10544)

Immunohistochemistry analysis of paraffin-embedded mouse lung using VDAC3 Rabbit pAb (A10544) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - VDAC3 Rabbit pAb (A10544)}

Immunofluorescence - VDAC3 Rabbit pAb (A10544)

Immunofluorescence analysis of HeLa cells using VDAC3 Rabbit pAb (A10544) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)