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KIF22 Rabbit pAb (A15285)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - KIF22 Rabbit pAb (A15285)

Western blot analysis of various lysates using KIF22 Rabbit pAb (A15285) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - KIF22 Rabbit pAb (A15285)

Immunohistochemistry analysis of paraffin-embedded Human colon using KIF22 Rabbit pAb (A15285) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - KIF22 Rabbit pAb (A15285)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using KIF22 Rabbit pAb (A15285) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - KIF22 Rabbit pAb (A15285)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using KIF22 Rabbit pAb (A15285) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameKIF22 Rabbit pAb
Catalog No.A15285
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 456-665 of human KIF22 (NP_015556.1).
SequenceQGAPLLSTPKRERMVLMKTVEEKDLEIERLKTKQKELEAKMLAQKAEEKENHCPTMLRPLSHRTVTGAKPLKKAVVMPLQLIQEQAASPNAEIHILKNKGRKRKLESLDALEPEEKAEDCWELQISPELLAHGRQKILDLLNEGSARDLRSLQRIGPKKAQLIVGWRELHGPFSQVEDLERVEGITGKQMESFLKANILGLAAGQRCGAS
Gene ID
Swiss Prot
SynonymsKID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2; KIF22
Calculated MW73kDa
Observed MW73kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA431, 293T, U-87MG, A549, HeLa, Jurkat, Mouse testis
Cellular locationCytoplasm, Nucleus, cytoskeleton.

Documents

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ABclonal:Western blot - KIF22 Rabbit pAb (A15285)}

Western blot - KIF22 Rabbit pAb (A15285)

Western blot analysis of various lysates using KIF22 Rabbit pAb (A15285) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - KIF22 Rabbit pAb (A15285)}

Immunohistochemistry - KIF22 Rabbit pAb (A15285)

Immunohistochemistry analysis of paraffin-embedded Human colon using KIF22 Rabbit pAb (A15285) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - KIF22 Rabbit pAb (A15285)}

Immunohistochemistry - KIF22 Rabbit pAb (A15285)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using KIF22 Rabbit pAb (A15285) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - KIF22 Rabbit pAb (A15285)}

Immunohistochemistry - KIF22 Rabbit pAb (A15285)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using KIF22 Rabbit pAb (A15285) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)