제품 > 항체 > 단일클론항체(mAb)

KIF23 Rabbit mAb (A4896)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates using KIF23 Rabbit mAb (A4896) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - KIF23 Rabbit mAb (A4896)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KIF23 antibody (A4896). Western blot was performed from the immunoprecipitate using KIF23 antibody (A4896) at a dilution of 1:1000.

Overview

Product name	KIF23 Rabbit mAb
Catalog No.	A4896
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0335

Background

The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 801-960 of human KIF23 (Q02241).
Sequence	NAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP
Gene ID
Swiss Prot
Synonyms	CDA3; CHO1; CDAN3; KNSL5; MKLP1; CDAIII; CDAN3A; MKLP-1; KIF23
Calculated MW	110kDa
Observed MW	115kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	HeLa, Rat testis
Cellular location	Cytoplasm, Midbody, Nucleus, cytoskeleton, spindle
Customer validation	WB(Rattus norvegicus, Homo sapiens)