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KIR3DS1 Rabbit pAb (A9936)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KIR3DS1 Rabbit pAb (A9936)

Western blot analysis of various lysates using KIR3DS1 Rabbit pAb (A9936) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - KIR3DS1 Rabbit pAb (A9936)

Immunohistochemistry analysis of paraffin-embedded Human liver using KIR3DS1 Rabbit pAb (A9936) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - KIR3DS1 Rabbit pAb (A9936)

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using KIR3DS1 Rabbit pAb (A9936) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameKIR3DS1 Rabbit pAb
Catalog No.A9936
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human KIR3DS1 (NP_001077008.1).
SequenceFNMSPVTTAHAGNYTCRGSHPHSPTGWSAPSNPMVIMVTGNHRKPSLLAHPGPLVKSGERVILQCWSDIMFEHFFLHKEWISKDPSRLVGQIHDGVSKANFSIGSMMRALAGTYRCYGSVTHTPYQLSAPSDPLDIVVTGLYEKPSLSAQPGPKVQAGESVTLSCSSRSSYDMYHLSREGGAHERRLPAVRKVNRTFQADFPLGPATHGGTYRCFGSFRHSPYEWSDPSDPLLVSVTGNPSSSWPSPTEPSSKSGNLRHLH
Gene ID
Swiss Prot
SynonymsKIR-G1; NKAT10; CD158E2; NKAT-10; KIR-123FM; KIR3DS1
Calculated MW42kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, A-549, THP-1, BT-474, B cells, Mouse liver, Mouse kidney, Mouse pancreas, Rat liver
Cellular locationCell membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - KIR3DS1 Rabbit pAb (A9936)}

    Western blot - KIR3DS1 Rabbit pAb (A9936)

    Western blot analysis of various lysates using KIR3DS1 Rabbit pAb (A9936) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - KIR3DS1 Rabbit pAb (A9936)}

    Immunohistochemistry - KIR3DS1 Rabbit pAb (A9936)

    Immunohistochemistry analysis of paraffin-embedded Human liver using KIR3DS1 Rabbit pAb (A9936) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - KIR3DS1 Rabbit pAb (A9936)}

    Immunohistochemistry - KIR3DS1 Rabbit pAb (A9936)

    Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using KIR3DS1 Rabbit pAb (A9936) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)