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KLF6 Rabbit pAb (A10011)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - KLF6 Rabbit pAb (A10011)

Western blot analysis of lysates from PC-3 cells, using KLF6 Rabbit pAb (A10011) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - KLF6 Rabbit pAb (A10011)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using KLF6 Rabbit pAb (A10011) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - KLF6 Rabbit pAb (A10011)

Immunofluorescence analysis of NIH/3T3 cells using KLF6 Rabbit pAb (A10011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKLF6 Rabbit pAb
Catalog No.A10011
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-200 of human KLF6 (NP_001291.3).
SequenceERYLQSEPCYVSASEIKFDSQEDLWTKIILAREKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELSPTAKFTSDPIGEVLVSSGKLSSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTSGKPGDKGNGDASPDGRRRVH
Gene ID
Swiss Prot
SynonymsGBF; ZF9; BCD1; CBA1; CPBP; PAC1; ST12; COPEB; KLF6
Calculated MW32kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesPC-3
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - KLF6 Rabbit pAb (A10011)}

Western blot - KLF6 Rabbit pAb (A10011)

Western blot analysis of lysates from PC-3 cells, using KLF6 Rabbit pAb (A10011) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - KLF6 Rabbit pAb (A10011)}

Immunohistochemistry - KLF6 Rabbit pAb (A10011)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using KLF6 Rabbit pAb (A10011) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - KLF6 Rabbit pAb (A10011)}

Immunofluorescence - KLF6 Rabbit pAb (A10011)

Immunofluorescence analysis of NIH/3T3 cells using KLF6 Rabbit pAb (A10011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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