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KLHDC2 Rabbit pAb (A15146)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - KLHDC2 Rabbit pAb (A15146)

Western blot analysis of lysates from K-562 cells, using KLHDC2 Rabbit pAb (A15146) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 150s.

ABclonal:Immunohistochemistry - KLHDC2 Rabbit pAb (A15146)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using KLHDC2 Rabbit pAb (A15146) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KLHDC2 Rabbit pAb (A15146)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using KLHDC2 Rabbit pAb (A15146) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Overview

Product nameKLHDC2 Rabbit pAb
Catalog No.A15146
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ubiquitin ligase-substrate adaptor activity. Involved in ubiquitin-dependent protein catabolic process via the C-end degron rule pathway. Located in nuclear body and nuclear membrane. Is active in Cul2-RING ubiquitin ligase complex and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 306-406 of human KLHDC2 (NP_055130.1).
SequenceEWIQFNHPYTEKPRLWHTACASDEGEVIVFGGCANNLLVHHRAAHSNEILIFSVQPKSLVRLSLEAVICFKEMLANSWNCLPKHLLHSVNQRFGSNNTSGS
Gene ID
Swiss Prot
SynonymsLCP; HCLP1; HCLP-1; KLHDC2
Calculated MW46kDa
Observed MW46kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562
Cellular locationNucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - KLHDC2 Rabbit pAb (A15146)}

    Western blot - KLHDC2 Rabbit pAb (A15146)

    Western blot analysis of lysates from K-562 cells, using KLHDC2 Rabbit pAb (A15146) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 150s.
    ABclonal:Immunohistochemistry - KLHDC2 Rabbit pAb (A15146)}

    Immunohistochemistry - KLHDC2 Rabbit pAb (A15146)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using KLHDC2 Rabbit pAb (A15146) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KLHDC2 Rabbit pAb (A15146)}

    Immunohistochemistry - KLHDC2 Rabbit pAb (A15146)

    Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using KLHDC2 Rabbit pAb (A15146) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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