제품 > 항체 > 다중클론항체(pAb)

KLHL1 Rabbit pAb (A14594)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - KLHL1 Rabbit pAb (A14594)

Western blot analysis of lysates from A-431 cells, using KLHL1 Rabbit pAb (A14594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameKLHL1 Rabbit pAb
Catalog No.A14594
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The KLHL1 protein belongs to a family of actin-organizing proteins related to Drosophila Kelch (Nemes et al., 2000 [PubMed 10888605]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human KLHL1 (NP_065917.1).
SequenceMSGSGRKDFDVKHILRLRWKLFSHPSPSTGGPAGGGCLQQDGSGSFEHWGPSQSRLLKSQERSGVSTFWKKPSSSSSSSSSPSSSSSSFNPLNGTLLPVATRLQQGAPGQGTQQPARTLFYVESLEEEVVPGMDFPGPHEKGLVLQELKVEPDNSSQATGEGCGHRLSSTGHSMTPQSDLDSSSSEEFYQAVHHAEQTFRKMESYLKQQQ
Gene ID
Swiss Prot
SynonymsMRP2; KLHL1
Calculated MW83kDa
Observed MW83kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-431
Cellular locationCytoplasm, cytoskeleton
ABclonal:Western blot - KLHL1 Rabbit pAb (A14594)}

Western blot - KLHL1 Rabbit pAb (A14594)

Western blot analysis of lysates from A-431 cells, using KLHL1 Rabbit pAb (A14594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)