제품 > 항체 > 다중클론항체(pAb)

KLHL3 Rabbit pAb (A13771)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - KLHL3 Rabbit pAb (A13771)

Western blot analysis of various lysates using KLHL3 Rabbit pAb (A13771) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameKLHL3 Rabbit pAb
Catalog No.A13771
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human KLHL3 (NP_059111.2).
SequenceMEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKVMNELRSKQLLCDVMIVAEDVEIEAHRVVLAACSPYFCAMFTGDMSESKAKKIEIKDVDGQTLSKLIDYIYT
Gene ID
Swiss Prot
SynonymsPHA2D; KLHL3
Calculated MW65kDa
Observed MW75kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, A-549, Mouse liver
Cellular locationCytoplasm, cytoskeleton, cytosol
    ABclonal:Western blot - KLHL3 Rabbit pAb (A13771)}

    Western blot - KLHL3 Rabbit pAb (A13771)

    Western blot analysis of various lysates using KLHL3 Rabbit pAb (A13771) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)