제품 > 항체 > 다중클론항체(pAb)

KLHL41 Rabbit pAb (A15383)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KLHL41 Rabbit pAb (A15383)

Western blot analysis of various lysates using KLHL41 Rabbit pAb (A15383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameKLHL41 Rabbit pAb
Catalog No.A15383
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the kelch-like family. The encoded protein contains a BACK domain, a BTB/POZ domain, and 5 Kelch repeats. This protein is thought to function in skeletal muscle development and maintenance. Mutations in this gene have been associated with nemaline myopathy (NM), a rare congenital muscle disorder.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KLHL41 (NP_006054.2).
SequenceMDSQRELAEELRLYQSTLLQDGLKDLLDEKKFIDCTLKAGDKSLPCHRLILSACSPYFREYFLSEIDEAKKKEVVLDNVDPAILDLIIKYLYSASIDLNDGNVQDIFALASRFQIPSVFTVCVSYLQKRLAPGNCLAILRLGLLLDCPRLAISAREFVSDRFVQICKEEDFMQLSPQELISVISNDSLNVEKEEAVFEAVMKWVRTDKENRVKNLSEVFDCIRFRLMTEKYFKDHVEKDDIIKSNPDLQKKIKVLKDAFAGKLPEPSKNAAKTGAGEVNGDVGDEDLLPGYLNDIPRHGM
Gene ID
Swiss Prot
SynonymsKrp1; KBTBD10; SARCOSIN; KLHL41
Calculated MW68kDa
Observed MW68kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, Mouse heart, Mouse skeletal muscle, Rat skeletal muscle
Cellular locationCell projection, Cytoplasm, Endoplasmic reticulum membrane, M line, Sarcoplasmic reticulum membrane, cytoskeleton, myofibril, pseudopodium, ruffle, sarcomere
    ABclonal:Western blot - KLHL41 Rabbit pAb (A15383)}

    Western blot - KLHL41 Rabbit pAb (A15383)

    Western blot analysis of various lysates using KLHL41 Rabbit pAb (A15383) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)