제품 > 항체 > 다중클론항체(pAb)

KLHL7 Rabbit pAb (A7817)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using KLHL7 Rabbit pAb (A7817) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	KLHL7 Rabbit pAb
Catalog No.	A7817
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a BTB-Kelch-related protein. The encoded protein may be involved in protein degradation. Mutations in this gene have been associated with retinitis pigmentosa 42.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human KLHL7 (NP_061334.4).
Sequence	QAEPLIQDNPECLKMVISGMRYHLLSPEDREELVDGTRPRRKKHDYRIALFGGSQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFP
Gene ID
Swiss Prot
Synonyms	CISS3; KLHL6; SBBI26; PERCHING; KLHL7
Calculated MW	66kDa
Observed MW	66kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431, Mouse testis, Mouse thymus, Mouse brain
Cellular location	Nucleus
Customer validation	WB(Homo sapiens)