제품 > 항체 > 다중클론항체(pAb)

KLHL8 Rabbit pAb (A14917)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KLHL8 Rabbit pAb (A14917)

Western blot analysis of various lysates using KLHL8 Rabbit pAb (A14917) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - KLHL8 Rabbit pAb (A14917)

Immunofluorescence analysis of C6 cells using KLHL8 Rabbit pAb (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KLHL8 Rabbit pAb (A14917)

Immunofluorescence analysis of L929 cells using KLHL8 Rabbit pAb (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KLHL8 Rabbit pAb (A14917)

Immunofluorescence analysis of U-2 OS cells using KLHL8 Rabbit pAb (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKLHL8 Rabbit pAb
Catalog No.A14917
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in protein ubiquitination and ubiquitin-dependent protein catabolic process. Located in nucleoplasm. Part of Cul3-RING ubiquitin ligase complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 271-620 of human KLHL8 (NP_065854.3).
SequenceAKEQIVKQNLKCRDLLDEARNYHLHLSSRAVPDFEYSIRTTPRKHTAGVLFCVGGRGGSGDPFRSIECYSINKNSWFFGPEMNSRRRHVGVISVEGKVYAVGGHDGNEHLGSMEMFDPLTNKWMMKASMNTKRRGIALASLGGPIYAIGGLDDNTCFNDVERYDIESDQWSTVAPMNTPRGGVGSVALVNHVYAVGGNDGMASLSSVERYDPHLDKWIEVKEMGQRRAGNGVSKLHGCLYVVGGFDDNSPLSSVERYDPRSNKWDYVAALTTPRGGVGIATVMGKIFAVGGHNGNAYLNTVEAFDPVLNRWELVGSVSHCRAGAGVAVCSCLTSQIRDVGHGSNNVVDCM
Gene ID
Swiss Prot
SynonymsKLHL8
Calculated MW69kDa
Observed MW69kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, Mouse brain, Mouse kidney, Rat thymus
Cellular locationnucleoplasm

Documents

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ABclonal:Western blot - KLHL8 Rabbit pAb (A14917)}

Western blot - KLHL8 Rabbit pAb (A14917)

Western blot analysis of various lysates using KLHL8 Rabbit pAb (A14917) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - KLHL8 Rabbit pAb (A14917)}

Immunofluorescence - KLHL8 Rabbit pAb (A14917)

Immunofluorescence analysis of C6 cells using KLHL8 Rabbit pAb (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KLHL8 Rabbit pAb (A14917)}

Immunofluorescence - KLHL8 Rabbit pAb (A14917)

Immunofluorescence analysis of L929 cells using KLHL8 Rabbit pAb (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KLHL8 Rabbit pAb (A14917)}

Immunofluorescence - KLHL8 Rabbit pAb (A14917)

Immunofluorescence analysis of U-2 OS cells using KLHL8 Rabbit pAb (A14917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)