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KLK10 Rabbit pAb (A6398)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KLK10 Rabbit pAb (A6398)

Western blot analysis of various lysates using KLK10 Rabbit pAb (A6398) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - KLK10 Rabbit pAb (A6398)

Immunofluorescence analysis of MCF7 cells using KLK10 Rabbit pAb (A6398) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KLK10 Rabbit pAb (A6398)

Immunofluorescence analysis of NIH/3T3 cells using KLK10 Rabbit pAb (A6398) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KLK10 Rabbit pAb (A6398)

Immunofluorescence analysis of PC-3 cells using KLK10 Rabbit pAb (A6398) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKLK10 Rabbit pAb
Catalog No.A6398
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 43-276 of human KLK10 (NP_665895.1).
SequenceLDPEAYGSPCARGSQPWQVSLFNGLSFHCAGVLVDQSWVLTAAHCGNKPLWARVGDDHLLLLQGEQLRRTTRSVVHPKYHQGSGPILPRRTDEHDLMLLKLARPVVLGPRVRALQLPYRCAQPGDQCQVAGWGTTAARRVKYNKGLTCSSITILSPKECEVFYPGVVTNNMICAGLDRGQDPCQSDSGGPLVCDETLQGILSWGVYPCGSAQHPAVYTQICKYMSWINKVIRSN
Gene ID
Swiss Prot
SynonymsNES1; PRSSL1; KLK10
Calculated MW30kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver, Mouse brain, Mouse heart, Rat liver
Cellular locationSecreted

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ABclonal:Western blot - KLK10 Rabbit pAb (A6398)}

Western blot - KLK10 Rabbit pAb (A6398)

Western blot analysis of various lysates using KLK10 Rabbit pAb (A6398) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - KLK10 Rabbit pAb (A6398)}

Immunofluorescence - KLK10 Rabbit pAb (A6398)

Immunofluorescence analysis of MCF7 cells using KLK10 Rabbit pAb (A6398) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KLK10 Rabbit pAb (A6398)}

Immunofluorescence - KLK10 Rabbit pAb (A6398)

Immunofluorescence analysis of NIH/3T3 cells using KLK10 Rabbit pAb (A6398) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - KLK10 Rabbit pAb (A6398)}

Immunofluorescence - KLK10 Rabbit pAb (A6398)

Immunofluorescence analysis of PC-3 cells using KLK10 Rabbit pAb (A6398) at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)