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KLRC4 Rabbit pAb (A14807)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KLRC4 Rabbit pAb (A14807)

Western blot analysis of various lysates using KLRC4 Rabbit pAb (A14807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - KLRC4 Rabbit pAb (A14807)

Immunofluorescence analysis of Jurkat cells using KLRC4 Rabbit pAb (A14807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - KLRC4 Rabbit pAb (A14807)

Immunofluorescence analysis of paraffin-embedded mouse spleen using KLRC4 Rabbit pAb (A14807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKLRC4 Rabbit pAb
Catalog No.A14807
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene is a member of the NKG2 group of genes that are expressed primarily in natural killer (NK) cells. These family members encode transmembrane proteins that are characterized by a type II membrane orientation (have an extracellular C-terminus) and the presence of a C-type lectin domain. This family member is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. Read-through transcription exists between this gene and the downstream KLRK1 (killer cell lectin-like receptor subfamily K, member 1) family member.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-55 of human KLRC4 (NP_038459.1).
SequenceMNKQRGTYSEVSLAQDPKRQQRKLKGNKISISGTKQEIFQVELNLQNASSDHQGN
Gene ID
Swiss Prot
SynonymsNKG2F; NKG2-F; KLRC4
Calculated MW18kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-549, NCI-H460, 293T, Mouse lung, Mouse kidney, Rat kidney
Cellular locationMembrane, Single-pass type II membrane protein

Documents

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    ABclonal:Western blot - KLRC4 Rabbit pAb (A14807)}

    Western blot - KLRC4 Rabbit pAb (A14807)

    Western blot analysis of various lysates using KLRC4 Rabbit pAb (A14807) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - KLRC4 Rabbit pAb (A14807)}

    Immunofluorescence - KLRC4 Rabbit pAb (A14807)

    Immunofluorescence analysis of Jurkat cells using KLRC4 Rabbit pAb (A14807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - KLRC4 Rabbit pAb (A14807)}

    Immunofluorescence - KLRC4 Rabbit pAb (A14807)

    Immunofluorescence analysis of paraffin-embedded mouse spleen using KLRC4 Rabbit pAb (A14807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)