제품 > 항체 > 다중클론항체(pAb)

KL Rabbit pAb (A12028)

Publications (11) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KL Rabbit pAb (A12028)

Western blot analysis of various lysates using KL Rabbit pAb (A12028) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameKL Rabbit pAb
Catalog No.A12028
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KL (NP_004786.2).
SequenceMPASAPPRRPRPPPPSLSLLLVLLGLGGRRLRAEPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRL
Gene ID
Swiss Prot
SynonymsKLA; HFTC3; KL
Calculated MW116kDa
Observed MW65kDa, 120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesDU145, Mouse kidney, Mouse brain, Rat kidney
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Homo sapiens)

IHC(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - KL Rabbit pAb (A12028)}

    Western blot - KL Rabbit pAb (A12028)

    Western blot analysis of various lysates using KL Rabbit pAb (A12028) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A12028? Please let us know so that we can cite the reference in this datasheet.

    ELISA 키트 (1)

    Secondary Antibodies (26)