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[KO Validated] ANKRD52 Rabbit pAb (A19958)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] ANKRD52 Rabbit pAb (A19958)

Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit pAb (A19958) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KO Validated] ANKRD52 Rabbit pAb
Catalog No.A19958
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Putative regulatory subunit of protein phosphatase 6 (PP6 that may be involved in the recognition of phosphoprotein substrates.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 450-660 of human ANKRD52 (NP_775866.2).
SequenceRRRDKFGRTPLHYAAANGSYQCAVTLVTAGAGVNEADCKGCSPLHYAAASDTYRRAEPHTPSSHDAEEDEPLKESRRKEAFFCLEFLLDNGADPSLRDRQGYTAVHYAAAYGNRQNLELLLEMSFNCLEDVESTIPVSPLHLAAYNGHCEALKTLAETLVNLDVRDHKGRTALFLATERGSTECVEVLTAHGASALIKERKRKWTPLHAAA
Gene ID
Swiss Prot
SynonymsANKRD33; 52
Calculated MW115kDa
Observed MW115kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular location
    ABclonal:Western blot - [KO Validated] ANKRD52 Rabbit pAb (A19958)}

    Western blot - [KO Validated] ANKRD52 Rabbit pAb (A19958)

    Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit pAb (A19958) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (2)

    Secondary Antibodies (26)