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[KO Validated] BRD7 Rabbit pAb (A2308)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] BRD7 Rabbit pAb (A2308)

Western blot analysis of various lysates using [KO Validated] BRD7 Rabbit pAb (A2308) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] BRD7 Rabbit pAb (A2308)

Western blot analysis of lysates from wild type (WT) and BRD7 knockout (KO) 293T cells, using [KO Validated] BRD7 Rabbit pAb (A2308) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name[KO Validated] BRD7 Rabbit pAb
Catalog No.A2308
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human BRD7 (NP_037395.2).
SequenceMGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRD
Gene ID
Swiss Prot
SynonymsBP75; NAG4; CELTIX1; SMARCI1; D7
Calculated MW74kDa
Observed MW100kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, HepG2, SW620, 293T
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - [KO Validated] BRD7 Rabbit pAb (A2308)}

    Western blot - [KO Validated] BRD7 Rabbit pAb (A2308)

    Western blot analysis of various lysates using [KO Validated] BRD7 Rabbit pAb (A2308) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - [KO Validated] BRD7 Rabbit pAb (A2308)}

    Western blot - [KO Validated] BRD7 Rabbit pAb (A2308)

    Western blot analysis of lysates from wild type (WT) and BRD7 knockout (KO) 293T cells, using [KO Validated] BRD7 Rabbit pAb (A2308) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)