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[KO Validated] Beclin 1 Rabbit mAb (A21191)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (A21191) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of extracts of Rat lung, using Beclin 1 antibody (A21191) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Western blot analysis of extracts from wild type (WT) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (A21191) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KO Validated] Beclin 1 Rabbit mAb (A21191)

Confocal imaging of HeLa cells using [KO Validated] Beclin 1 Rabbit mAb (A21191, dilution 1:100) (Red). The cells were counterstained with [KO Validated] Vimentin Rabbit mAb (A19607, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product name	[KO Validated] Beclin 1 Rabbit mAb
Catalog No.	A21191
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC52856

Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Sequence	MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSG
Gene ID
Swiss Prot
Synonyms	ATG6; VPS30; beclin1; Beclin 1; BECN1; beclin-1
Calculated MW	52kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T, HeLa, Jurkat, Mouse spleen, Mouse testis, Rat lung
Cellular location
Customer validation	WB(Homo sapiens, Mus musculus, Mus musculus , Dianella ensifolia, Rattus norvegicus, Other, Sus scrofa) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구세포 생물학 & 발달 생물학자가포식조절자

[KO Validated] Beclin 1 Rabbit mAb images

Western blot - [KO Validated] Beclin 1 Rabbit mAb (A21191)

Immunofluorescence - [KO Validated] Beclin 1 Rabbit mAb (A21191)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

JCI insight

Application:

IF:

9.48

Species:

Homo sapiens

PMID:

36166295

Title:

Interleukin-37 protects against acinar cell pyroptosis in acute pancreatitis

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.67

Species:

Mus musculus

PMID:

35739966

Title:

Alanyl-Glutamine Protects against Lipopolysaccharide-Induced Liver Injury in Mice via Alleviating Oxidative Stress, Inhibiting Inflammation, and Regulating Autophagy

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

International journal of medical sciences

Application:

IF:

3.64

Species:

Homo sapiens

PMID:

37082736

Title:

SIRT6 ameliorates LPS-induced apoptosis and tight junction injury in ARDS through the ERK1/2 pathway and autophagy - PMC

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi

Application:

IF:

10.27

Species:

Mus musculus

PMID:

37147244

Title:

The therapeutic effect of Tanshinone IIA in mouse astrocytes after treatment with Angiostrongylus cantonensis fifth-stage larval excretory-secretory products

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Journal of Cancer

Application:

IF:

4.47

Species:

Dianella ensifolia,Homo sapiens

PMID:

38164280

Title:

Dimethoxytolyl propylresorcinol induces apoptosis and mitophagy in human leukemia cells through the PI3K/AKT pathway - PMC

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Homo sapiens

PMID:

38398837

Title:

2-Hydroxychalcone Induces Autophagy and Apoptosis in Breast Cancer Cells via the Inhibition of the NF-B Signaling Pathway: In Vitro and In Vivo Studies - PMC

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Biochemical pharmacology

Application:

IF:

6.1

Species:

Rattus norvegicus

PMID:

37863322

Title:

20-Deoxyingenol alleviates intervertebral disc degeneration by activating TFEB in nucleus pulposus cells

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Clinical and experimental pharmacology & physiology

Application:

WB, IF

IF:

2.96

Species:

Rattus norvegicus

PMID:

37582493

Title:

Aucubin alleviates methotrexateinduced enteritis in rats by inducing autophagy

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Other

PMID:

38594767

Title:

Csn5 inhibits autophagy by regulating the ubiquitination of Atg6 and Tor to mediate the pathogenicity of Magnaporthe oryzae - PMC

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

38891873

Title:

Enhancing Heart Transplantation: Utilizing Gas-Loaded Nanocarriers to Mitigate Cold/Hypoxia Stress - PMC

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

bioRxiv : the preprint server for biology

Application:

IF:

Species:

Mus musculus

PMID:

38895352

Title:

TRIM32 inhibits Venezuelan Equine Encephalitis Virus Infection by targeting a late step in viral entry - PMC

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Rattus norvegicus

PMID:

39143545

Title:

A structured biomimetic nanoparticle as inflammatory factor sponge and autophagy-regulatory agent against intervertebral disc degeneration and discogenic pain - PMC

References for [KO Validated] Beclin 1 Rabbit mAb (A21191)

Product:

[KO Validated] Beclin 1 Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

Species:

Sus scrofa

PMID:

38247545

Title: