Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of various lysates using [KO Validated] CD168/RHAMM Rabbit pAb (A12445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of lysates from wild type (WT) and CD168/RHAMM knockout (KO) HeLa cells, using [KO Validated] CD168/RHAMM Rabbit pAb (A12445) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
| Product name | [KO Validated] CD168/RHAMM Rabbit pAb |
|---|---|
| Catalog No. | A12445 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1). |
|---|---|
| Sequence | MSFPKAPLKRFNDPSGCAPSPGAYDVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDKDTTLPASARKVKSSESKKESQKNDKDLKILEKEIRVLLQERGAQDRRIQDLETELEKMEARLNAALREKTSLSANNATLEKQLIELTRTNELLKSKFSENGNQKNLRILSLELMKLRNKRETKMRGMMAKQEGMEMKLQVTQRSLEESQGKIAQLEGKLVSIEKEKIDEKSETEKLLEYIEEISCASDQVEKYKLDIAQLEENLKEKNDEILSLKQSLEENIVILSKQVEDLNVKCQLLEKE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CD168; IHABP; RHAMM; MM |
| Calculated MW | 84kDa |
| Observed MW | 84kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | SW480, HeLa, Jurkat, ES-2 |
| Cellular location | Cell surface, Cytoplasm |
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![ABclonal:Western blot - [KO Validated] CD168/RHAMM Rabbit pAb (A12445)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A12445_P433_WB_01.jpg?t=1708654261 "ABclonal:Western blot - [KO Validated] CD168/RHAMM Rabbit pAb (A12445)")
![ABclonal:Western blot - [KO Validated] CD168/RHAMM Rabbit pAb (A12445)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A12445_P434_KO-WB_01.jpg?t=1708654261 "ABclonal:Western blot - [KO Validated] CD168/RHAMM Rabbit pAb (A12445)")
![ABclonal:Immunofluorescence - [KO Validated] CD168/RHAMM Rabbit pAb (A12445)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A12445_P433_IF_01.jpg?t=1708654261 "ABclonal:Immunofluorescence - [KO Validated] CD168/RHAMM Rabbit pAb (A12445)")
