제품 > 항체 > 단일클론항체(mAb)

[KO Validated] Cleaved Gasdermin E (N terminal) Rabbit mAb (A26197)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from wild type (WT) and Gasdermin E (N terminal) knockout (KO) HeLa cells using [KO Validated] Cleaved Gasdermin E (N terminal) Rabbit mAb (A26197) at 1:2000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and GSDME knockout (KO) HeLa cells were treated by TNF-α (50ng/mL) and CHX (10μg/mL) at 37℃ for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Review

Overview

Product name	[KO Validated] Cleaved Gasdermin E (N terminal) Rabbit mAb
Catalog No.	A26197
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC70388

Background

Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to residues surrounding Asp270 of human Gasdermin E(NP_004394.1).
Sequence	IDMPD
Gene ID
Swiss Prot
Synonyms	DFNA5; ICERE-1
Calculated MW	55kDa
Observed MW	35kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa cells were treated by TNF-α and CHX
Cellular location	Cytoplasm, Cytosol, Plasma membrane.
Customer validation	(HeLa cells)