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[KO Validated] EDN1 Rabbit pAb (A18004)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] EDN1 Rabbit pAb (A18004)

Western blot analysis of lysates from wild type (WT) and EDN1 knockout (KO) HeLa cells, using [KO Validated] EDN1 Rabbit pAb (A18004) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Overview

Product name[KO Validated] EDN1 Rabbit pAb
Catalog No.A18004
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 18-212 of human EDN1 (NP_001946.3).
SequenceAPETAVLGAELSAVGENGGEKPTPSPPWRLRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHVVPYGLGSPRSKRALENLLPTKATDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQLVRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGKPSRERYVTHNRAHW
Gene ID
Swiss Prot
SynonymsET1; QME; PPET1; ARCND3; HDLCQ7; N1
Calculated MW24kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationSecreted.
    ABclonal:Western blot - [KO Validated] EDN1 Rabbit pAb (A18004)}

    Western blot - [KO Validated] EDN1 Rabbit pAb (A18004)

    Western blot analysis of lysates from wild type (WT) and EDN1 knockout (KO) HeLa cells, using [KO Validated] EDN1 Rabbit pAb (A18004) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1min.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)