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[KO Validated] FH Rabbit pAb (A21403)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] FH Rabbit pAb (A21403)

Western blot analysis of various lysates, using FH Rabbit pAb (A21403) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] FH Rabbit pAb (A21403)

Western blot analysis of extracts from wild type(WT) and FH knockout (KO) 293T(KO) cells, using FH antibody (A21403) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name[KO Validated] FH Rabbit pAb
Catalog No.A21403
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human FH (NP_000134.2).
SequenceFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTN
Gene ID
Swiss Prot
SynonymsMCL; FMRD; HsFH; LRCC; HLRCC; MCUL1
Calculated MW55kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Hela, NIH/3T3, C2C12, Rat liver
Cellular locationCytoplasm, Mitochondrion

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - [KO Validated] FH Rabbit pAb (A21403)}

    Western blot - [KO Validated] FH Rabbit pAb (A21403)

    Western blot analysis of various lysates, using FH Rabbit pAb (A21403) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - [KO Validated] FH Rabbit pAb (A21403)}

    Western blot - [KO Validated] FH Rabbit pAb (A21403)

    Western blot analysis of extracts from wild type(WT) and FH knockout (KO) 293T(KO) cells, using FH antibody (A21403) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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