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[KO Validated] GSTK1 Rabbit pAb (A21473)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] GSTK1 Rabbit pAb (A21473)

Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells using [KO Validated] GSTK1 Rabbit pAb (A21473) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] GSTK1 Rabbit pAb (A21473)

Western blot analysis of various lysates using [KO Validated] GSTK1 Rabbit pAb (A21473) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - [KO Validated] GSTK1 Rabbit pAb (A21473)

Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit pAb (A21473) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] GSTK1 Rabbit pAb
Catalog No.A21473
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1).
SequenceMGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL
Gene ID
Swiss Prot
SynonymsGST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13; GSTK1
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HeLa
Cellular locationPeroxisome.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] GSTK1 Rabbit pAb (A21473)}

Western blot - [KO Validated] GSTK1 Rabbit pAb (A21473)

Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells using [KO Validated] GSTK1 Rabbit pAb (A21473) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] GSTK1 Rabbit pAb (A21473)}

Western blot - [KO Validated] GSTK1 Rabbit pAb (A21473)

Western blot analysis of various lysates using [KO Validated] GSTK1 Rabbit pAb (A21473) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - [KO Validated] GSTK1 Rabbit pAb (A21473)}

Immunofluorescence - [KO Validated] GSTK1 Rabbit pAb (A21473)

Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit pAb (A21473) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)