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[KO Validated] HNRNPF Rabbit pAb (A5505)

KO/KDValidated

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] HNRNPF Rabbit pAb (A5505)

Western blot analysis of extracts of various cell lines, using HNRNPF antibody (A5505) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] HNRNPF Rabbit pAb (A5505)

Western blot analysis of extracts from wild type(WT) and HNRNPF knockout (KO) 293T cells, using HNRNPF antibody (A5505) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - [KO Validated] HNRNPF Rabbit pAb (A5505)

Immunofluorescence analysis of U2OS cells using HNRNPF antibody (A5505). Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] HNRNPF Rabbit pAb
Catalog No.A5505
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1).
SequenceMMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSE
Gene ID
Swiss Prot
SynonymsHNRPF; mcs94-1; OK/SW-cl.23
Calculated MW46kDa
Observed MW47kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples22Rv1, Lovo, MCF7, BT-474, Mouse spleen
Cellular locationNucleus, nucleoplasm
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] HNRNPF Rabbit pAb (A5505)}

Western blot - [KO Validated] HNRNPF Rabbit pAb (A5505)

Western blot analysis of extracts of various cell lines, using HNRNPF antibody (A5505) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] HNRNPF Rabbit pAb (A5505)}

Western blot - [KO Validated] HNRNPF Rabbit pAb (A5505)

Western blot analysis of extracts from wild type(WT) and HNRNPF knockout (KO) 293T cells, using HNRNPF antibody (A5505) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] HNRNPF Rabbit pAb (A5505)}

Immunofluorescence - [KO Validated] HNRNPF Rabbit pAb (A5505)

Immunofluorescence analysis of U2OS cells using HNRNPF antibody (A5505). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5505? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)