제품 > 항체 > 단일클론항체(mAb)

[KO Validated] HPRT1 Rabbit mAb (A8783)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] HPRT1 Rabbit mAb (A8783)

Western blot analysis of various lysates using HPRT11 Rabbit mAb (A8783) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] HPRT1 Rabbit mAb (A8783)

Western blot analysis of lysates from wild type(WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit mAb (A8783) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name[KO Validated] HPRT1 Rabbit mAb
Catalog No.A8783
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1300
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 119-218 of human HPRT11 (NP_000185.1).
SequenceGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA
Gene ID
Swiss Prot
SynonymsHPRT; HGPRT; T1
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, 293T, NIH/3T3, Mouse brain, Mouse heart, Rat heart, Rat thymus
Cellular locationCytoplasm.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KO Validated] HPRT1 Rabbit mAb (A8783)}

    Western blot - [KO Validated] HPRT1 Rabbit mAb (A8783)

    Western blot analysis of various lysates using HPRT11 Rabbit mAb (A8783) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - [KO Validated] HPRT1 Rabbit mAb (A8783)}

    Western blot - [KO Validated] HPRT1 Rabbit mAb (A8783)

    Western blot analysis of lysates from wild type(WT) and HPRT1 knockout (KO) 293T cells, using [KO Validated] HPRT1 Rabbit mAb (A8783) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    ELISA 키트 (1)

    Secondary Antibodies (26)